Protocols: Retroviral Infection
Media
- C10: Lymphocytes: RPMI 1640 + 10% FCS, L-glu, pen/strep, hepes, NEAA, Na-pyruvate and 2-BME
- D10: Lymphocytes: DMEM + 10% FCS, L-glu, pen/strep, hepes, NEAA, Na-pyruvate and 2-BME
- Bone Marrow Media: DMEM + 15% FCS, L-glu, pen/strep, IL-3, IL-6, SCF
Preparing T cells for Infection
- Plate 106 purified CD4+ T cells
- Activate with 1ug/ml of anti-CD3 and anti-CD28
- Infect after 24hrs
Preparing B cells for Infection
- Plate 106 purified CD4+ T cells
- Activate with 1ug/ml of anti-IgM and anti-CD40
- Infect after 24hrs