Mouse & Cell Lines
Mouse Lines
All of our mice are on a C57BL/6 background or are in the process of becoming so. We have several strains which overexpress MYC, either consitutively, in a B-cell or T-cell specific manner, or inducibly. We also have several mice for examining antigen receptors and their ligands.
E-mu MYC - Transgenics, express MYC off of the heavy-chain Ig promoter, C57BL/6 background.
Floxed MYC - Endogenous MYC allele flanked by loxP sites. Mixed Background.
TRE-MYC - TET-responsive MYC,currently backcrossing to C57BL/6
HEL-VDJ-Knock-in - HEL-specific VDJ knocked-into the endogenous IgH locus. C57BL/6 background.
BCR-HEL - Transgenics, C57BL/6 background. express a BCR specific for HEL
MD4 - HEL specific BCR transgene, C57BL/6
ML5 - Express soluble HEL, C57BL/6 background.
KLK4 - membrane-bound HEL, C57BL/6 background.
OTII TCR - Transgenics, TCR specific for Kb and an Ova peptide. C57BL/6 background.
MMTV-rtTA - B-cell specific TET-off repressor, currently backcrossing to C57BL/6
E-mu-rtTA - T-cell specific TET-off repressor, currently backcrossing to C57BL/6
YETI - YFP-enhanced transcript of IFN-gamma. C57BL/6 background.
P110-Delta Knock-in - Knock-in Point mutation for PI3K-P110-delta isoform (inactivated). C57BL/6 background.
Cell Lines
We have many cell lines derived from the T and B cell lymphomas and leukemias isolated from the spleens, lymph nodes and thymi of many of our different transgenic mice.
These include:
E-mu-Myc/MD4
Eu-Myc/MD4/MLS
MMTV-tTA/TRE-Myc/MD4
MMTV-tTA/TRE-Myc/MD4/MLS
E-mu-tTA/TRE-Myc
Myc-ER/hBL1-2
HSC’s